Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

LCP1 (D1C3) Rabbit mAb #3588

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M (Mk) Endogenous 70 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LCP1 (D1C3) Rabbit mAb recognizes endogenous levels of total LCP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp567 of human LCP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LCP1 (D1C3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LCP1 (D1C3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using LCP1 (D1C3) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using LCP1 (D1C3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon using LCP1 (D1C3) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Background

Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mammals and include L-plastin, T-plastin, and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues, while I-plastin (plastin-1) is expressed specifically in the kidney, colon, and small intestine (1-3). Research studies have shown that L-plastin (plastin-2) or lymphocyte cytosolic protein 1 (LCP1) is mainly expressed in hematopoietic cells and nonhematopoietic tumors, and increased expression correlates with metastatic progression in colon cancer cell lines (4). Investigators have found that overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T cell receptor/CD3 complex in association with the CD2 cell adhesion molecule or the CD28 receptor (6). Phosphorylation at Ser5 enhances the ability of LCP1 to bind to F-actin and increases cell motility (7,8).

  1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92.
  2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67.
  3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79.
  4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81.
  5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104.
  6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62.
  7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60.
  8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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