Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Rb (Ser780) (C84F6) Rabbit mAb #3590

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk (Pg) Endogenous 110 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Rb (Ser780) (C84F6) Rabbit mAb detects endogenous levels of total Rb protein only when phosphorylated at Ser780.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein.

Western Blotting

Western Blotting

Western blot analysis of recombinant truncated Rb, without or with Rb blocking peptide, using Phospho-Rb (Ser780) (C84F6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS and Jurkat cells using Phospho-Rb (Ser780) (C84F6) Rabbit mAb.

IP

IP

Immunoprecipitation of phospho-Rb (Ser780) from Jurkat cell lysates using Phospho-Rb (Ser780) (C84F6) Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input.


Background

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

  1. Sherr, C.J. (1996) Science 274, 1672-7.
  2. Nevins, J.R. (1992) Science 258, 424-9.
  3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
  4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
  5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
  6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
  7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
  8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
  9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

Application References

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Companion Products

Rabbit monclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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