Product Pathways - Translational Control
Phospho-eIF4B (Ser422) Antibody #3591
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 80 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-eIF4B (Ser422) Antibody detects eIF4B only when phosphorylated at Ser422.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser422 of human eIF4B. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody (upper) or eIF4B Antibody #3592 (lower). 48 hours following siRNA transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated.
Background
Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6).
p70 S6 Kinase has been shown to phosphorylate eIF4B at the rapamycin-sensitive site Ser422 in vivo, and a Ser422Ala mutant of eIF4B shows deminished activity in an in vitro translation assay (7).
- Le, H. et al. (1997) J. Biol. Chem. 272, 16247-16255.
- Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.
- Duncan, R.F. and Hershey, J.W. (1984) J. Biol. Chem. 259, 11882-11889.
- Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
- Manzella, J.M. et al. (1991) J. Biol. Chem. 266, 2383-2389.
- Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
- Raught, B. et al. (2004) EMBO J. 23, 1761-1769.
Application References
- Peiretti, F. et al. (2004) Serum- and mitogen-responsive phosphorylation of eukaryotic translation initiation factor 4B (eIF4B) Ser422 is modulated by ribosomal S6 kinases. EMBO J. 23, 1761-1769. This article references the use of Phospho-eIF4B (Ser422) Antibody in the following applications: Western Blotting
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Companion Products
- 3592 eIF4B Antibody
- 2708 p70 S6 Kinase (49D7) Rabbit mAb
- 9202 p70 S6 Kinase Antibody
- 4763 Translation Initiation Complex Antibody Sampler Kit
- 9904 Rapamycin (FRAP/mTOR Inhibitor)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
