Cell Signaling Technology

Product Pathways - Translational Control

eIF4B Antibody #3592

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF4B Antibody recognizes endogenous levels of eIF4B, independent of phosphorylation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human eIF4B. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, 293 and NIH/3T3 cells using eIF4B Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected or transfected with either nonspecific control siRNA or eIF4E siRNA. eIF4E was detected using eIF4E Antibody #9742, and eIF4B was detected using eIF4B Antibody #3592. The eIF4E Antibody confirms silencing of eIF4E expression, and the eIF4B Antibody is used to control for loading and siRNA specificity.

Background

Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translational activity of eIF4B (7).

  1. Le, H. et al. (1997) J. Biol. Chem. 272, 16247-16255.
  2. Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.
  3. Duncan, R.F. and Hershey, J.W. (1984) J. Biol. Chem. 259, 11882-11889.
  4. Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
  5. Manzella, J.M. et al. (1991) J. Biol. Chem. 266, 2383-2389.
  6. Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
  7. van Gorp, A.G. et al. (2009) Oncogene 28, 95-106.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products