Product Pathways - Translational Control
Grp75 (D13H4) XP® Rabbit mAb #3593
|W IP IHC-P IF-IC||H M Mk||Endogenous||75||Rabbit IgG|
Reactivity Key: H=Human M=Mouse Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Grp75 (D13H4) XP® Rabbit mAb detects endogenous levels of total Grp75 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Grp75.
Western blot analysis of extracts from various cell types using Grp75 (D13H4) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Grp75 (D13H4) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Grp75 (D13H4) XP® Rabbit mAb in the presence of a control peptide (left) or antigen-sprecific peptide (right).
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).
- Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55.
- Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21.
- Schneider, H.C. et al. (1994) Nature 371, 768-74.
- Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91.
- Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80.
- Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101.
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For Research Use Only. Not For Use In Diagnostic Procedures.