Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Translational Control

Grp75 (D13H4) XP® Rabbit mAb #3593

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H M Mk Endogenous 75 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Grp75 (D13H4) XP® Rabbit mAb detects endogenous levels of total Grp75 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Grp75.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Grp75 (D13H4) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Grp75 (D13H4) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Grp75 (D13H4) XP® Rabbit mAb in the presence of a control peptide (left) or antigen-sprecific peptide (right).


IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using Grp75 (D13H4) XP® Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).

  1. Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55.
  2. Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21.
  3. Schneider, H.C. et al. (1994) Nature 371, 768-74.
  4. Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91.
  5. Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80.
  6. Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products