Cell Signaling Technology

Product Pathways - Translational Control

Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H M R Mk Dm Endogenous 38 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-eIF2alpha (Ser51) RmAb detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 (human) and AR42J (mouse) cells, untreated or thapsigargin-treated (300 nM, 30 min), using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb (upper) or eIF2alpha Antibody #9722 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and THP-1 cells untreated or phosphatase treated, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.

IP

IP

Western blot analysis of immunoprecipitates from PC12 cells, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb. Lane 1 is lysate control, lane 2 is the antibody alone as negative control and lane 3 is antibody immunocomplex of PC12 cells.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded PC-3 cells untreated (left) or thapsigargin-treated (right), using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb in the presence of control peptide (left) or Phospho-eIF2alpha (Ser51) Blocking Peptide (#1221) (right).

Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

  1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
  2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
  3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
  4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
  5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
  6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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