Cell Signaling Technology

Product Pathways - Adhesion

EpCAM Antibody #3599

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

EpCAM Antibody detects endogenous levels of total EpCAM protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human EpCAM.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29, HCT-15 and HCT-116 cells using EpCAM Antibody.

Background

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).

  1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
  2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
  3. Armstrong, A. and Eck, S.L. Cancer Biol Ther 2, 320-6.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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