Cell Signaling Technology

Product Pathways - Adhesion

NCAM (CD56) Antibody #3606

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 140 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NCAM (CD56) Antibody detects endogenous levels of total NCAM protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 800 of human NCAM. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum and rat brain, using NCAM (CD56) Antibody.

Background

NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma and rhabdomyosarcoma (2). CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (3).

  1. Cunningham, B. A. et al. (1987) Science 236, 799-806.
  2. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918.
  3. Robertson, M.J. and Ritz, J. (1990) Blood 76, 2421-2438.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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