Product Pathways - Wnt / Hedgehog / Notch
Notch1 (D1E11) XP® Rabbit mAb #3608
PhosphoSitePlus® protein, site, and accession data: Notch 1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P | H M R | Endogenous | 120, 300 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3608:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
Notch1 (D1E11) XP® Rabbit mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~300 kDa) and the transmembrane/intracellular region NTM (~120 kDa).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2438 of human Notch1.
Western Blotting
Western blot analysis of total cell extract from various cell types using Notch1 (D1E11) XP® Rabbit mAb. The full-length (FL) Notch protein and the cleaved transmembrane/intracellular region (NTM) are indicated.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human inflammatory granulation tissue using Notch1 (D1E11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Notch1 (D1E11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded A2780 (left), Jurkat (center) and RL (right) cell pellets using Notch1 (D1E11) XP® Rabbit mAb. Both A2780 and Jurkat express Notch1, but only Jurkat cells have cleaved Notch1, while RL cells express very low or no Notch1.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon using Notch1 (D1E11) XP® Rabbit mAb.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
Application References
- Choi, P.S. et al. (2011) Proc Natl Acad Sci U S A , . Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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- 2155 Jagged1 (1C4) Rabbit mAb
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- 2210 Jagged2 (C23D2) Rabbit mAb
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- 4608 MAML1 Antibody
- 4618 MAML2 Antibody
- 2756 Numb (C29G11) Rabbit mAb
- 2761 Numb (C44B4) Rabbit mAb
- 3976 TACE Antibody
- 3268 Notch1 (C44H11) Rabbit mAb
- 3447 Notch1 (5B5) Rat mAb
- 3446 Notch3 (8G5) Rat mAb
- 3632 Nicastrin Antibody
- 2154 Musashi Antibody
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
