Product Pathways - Akt Signaling
Phospho-Tuberin/TSC2 (Tyr1571) Antibody #3614
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M Mk (H) | Endogenous | 200 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Tuberin/TSC2 (Tyr1571) Antibody detects endogenous levels of tuberin only when phosphorylated at tyrosine 1571. This antibody does not detect tuberin phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr1571 of human tuberin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated, PDGF and wortmannin-treated or PDGF and rapamycin-treated, using Phospho-Tuberin/TSC2 (Tyr1571) Antibody (top), Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 (middle) or Tuberin/TSC2 Antibody #3612 (bottom).
Background
Tuberin, a product of the tumor suppressor gene TSC2, is important in regulating cell proliferation and tumor development (1). Mutations in either TSC1 or TSC2 are responsible for tuberous sclerosis complex (TSC), an autosomal dominant disorder (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). Additionally, tuberin inhibits the mammalian target of rapamycin (mTOR), resulting in inhibition of p70 S6 kinase and activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and thus, inhibits translation (3,6-7).
- Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
- Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
- Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
- Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
- Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
- Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967.
Application References
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Companion Products
- 3611 Phospho-Tuberin/TSC2 (Thr1462) Antibody
- 3612 Tuberin/TSC2 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
