Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb #3617

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 200 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb detects endogenous levels of tuberin only when phosphorylated at threonine 1462.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr1462 of human tuberin.

Western Blotting

Western Blotting

Western blot analysis of extracts from l phosphatase-treated or PDGF treated NIH/3T3 cells, using Phospho- Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb (upper) or Tuberin Antibody (lower).

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

  1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
  3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
  4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
  5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
  6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
  7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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