Cell Signaling Technology

Product Pathways - Neuroscience

Presenilin 1 Antibody #3622

Applications Reactivity Sensitivity MW (kDa) Source
W IP H R Mk (M) Endogenous 22 (C-terminal fragment). 55 (full-length). Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Presenilin 1 Antibody detects endogenous levels of the carboxy-terminal fragment of presenilin 1 (~22 kDa). The antibody detects the full length protein (55k Da) to a lesser extent.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues arround valine 293 of human presenilin 1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, SK-N-MC and COS cells, using Presenilin 1 Antibody.

Background

Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin's associated γ-secretase activity for amyloid-β protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (2,3).

  1. Haass, C. and DeStrooper, B. (1999) Science 286, 916-919.
  2. Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178.
  3. Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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