Product Pathways - Cytoskeletal Signaling
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3623
|IF-IC F||H M R Mk Dm Z B (C)||Endogenous||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Dm=D. melanogaster Z=Zebrafish B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human β-tubulin.
Flow cytometric analysis of NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Tubulin (9F3) Rabbit mAb #2128.
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
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- 2146 β-Tubulin Antibody
- 2128 β-Tubulin (9F3) Rabbit mAb
- 2116 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate)
- 3624 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
Rabbbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.