Product Pathways - Chromatin Regulation / Epigenetics

• pathway
• application references
• companion products
• datasheet PDF
• MSDS PDF
• protocols

NUT (C52B1) Rabbit mAb #3625

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-F	H R (Mk)	Endogenous	150	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3625:

IHC / Paraffin*, Immunofluorescence, Immunoprecipitation, Western Blotting

* Product-specific protocol.

Specificity / Sensitivity

NUT (C52B1) Rabbit mAb detects endogenous levels of total NUT protein. The antibody also detects endogenous levels of the BRD4-NUT fusion protein found in NUT midline carcinoma (NMC).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the human NUT protein.

Background

Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the NUT gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2).

1. French, C.A. et al. (2003) Cancer Res 63, 304-7.
2. Haack, H. et al. (2009) Am J Surg Pathol 33, 984-91.
3. French, C.A. et al. (2008) Oncogene 27, 2237-42.

Application References

• Haack, H. et al. (2009) Am J Surg Pathol 33, 984-91. Applications: IHC-P (paraffin)
• Yan, J. et al. (2011) J Biol Chem 286, 27663-75. Applications: IF-IC (In Cells) Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8112 SignalStain^® Antibody Diluent
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

---

For Research Use Only. Not For Use In Diagnostic Procedures.