Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

VEGF Receptor 2 (55B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3627

Applications Reactivity Sensitivity Isotype
F H M Endogenous Rabbit IgG

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

VEGF Receptor 2 (55B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total VEGF receptor 2 protein. This antibody does not cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2. This antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells (blue) and HUVEC (green) using VEGF Receptor 2 (55B11) Rabbit mAb (Alexa Fluor® 488 Conjugate).

Description

This Cell Signaling Technology (CST) antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. The unconjugated VEGF Receptor 2 (55B11) Rabbit mAb #2479 reacts with human and mouse VEGF receptor 2 protein. CST expects that VEGF Receptor 2 (55B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize VEGF receptor 2 in these species.

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

  1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
  2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
  3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
  4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
  5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
  6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
  7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
  8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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