Cell Signaling Technology

Product Pathways - Neuroscience

Nicastrin Antibody #3632

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 110, 120 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nicastrin Antibody detects endogenous levels of Nicastrin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human Nicastrin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human (HeLa and SK-N-MC; lanes 1 and 2), monkey (COS; lane 3), mouse (Neuro 2A and L; lanes 4 and 5) and rat (PC12 and C6; lanes 6 and 7) cell lines, using Nicastrin Antibody.

Background

Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).

  1. Yu, G. et al. (2000) Nature 407, 48-54.
  2. Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725.
  3. Kopan, R. and Goate, A. (2002) Neuron 33, 321-324.
  4. Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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