Product Pathways - Neuroscience
Nicastrin Antibody #3632
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 110, 120 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Nicastrin Antibody detects endogenous levels of Nicastrin.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminus of human Nicastrin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from human (HeLa and SK-N-MC; lanes 1 and 2), monkey (COS; lane 3), mouse (Neuro 2A and L; lanes 4 and 5) and rat (PC12 and C6; lanes 6 and 7) cell lines, using Nicastrin Antibody.
Background
Nicastrin is a transmembrane glycoprotein serving as an essential component of the gamma-secretase complex (1,2). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex (3). Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to gamma-secretase to ensure the correct cleavage process (2,4).
- Yu, G. et al. (2000) Nature 407, 48-54.
- Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725.
- Kopan, R. and Goate, A. (2002) Neuron 33, 321-324.
- Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 2421 Cleaved Notch1 (Val1744) Antibody
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
