Product Pathways - Neuroscience
Nicastrin Antibody #3632
PhosphoSitePlus® protein, site, and accession data: nicastrin
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 110, 120 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3632:
- Western Blotting
Specificity / Sensitivity
Nicastrin Antibody detects endogenous levels of Nicastrin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human Nicastrin. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).
- Yu, G. et al. (2000) Nature 407, 48-54.
- Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725.
- Kopan, R. and Goate, A. (2002) Neuron 33, 321-324.
- Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2192 Presenilin 2 Antibody
- 3622 Presenilin 1 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2421 Cleaved Notch1 (Val1744) Antibody
- 3268 Notch1 (C44H11) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.