Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

PRC1 Antibody #3639

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 70 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PRC1 Antibody recognizes endogenous levels of total PRC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly522 of human PRC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PRC1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or nocodazole-treated (+; 100 ng/ml overnight) to induce mitotic arrest and increase CDK1 activity. Increased CDK1 activity induces PRC1 phosphorylation, as indicated by the upper band.

Background

Protein Regulator of Cytokinesis 1 (PRC1) is a member of the MAP65/ASE1 family of nonmotor microtubule-associated proteins, first described in budding yeast (1,2). PRC1 is a substrate of CDK1, which maintains PRC1 in an inactive, monomeric state (2). Cell-cycle dependent degradation of CDK1 leads to dephosphorylation of PRC1 and subsequent KIF4-mediated translocation to the plus ends of microtubules, where it promotes microtubule bundling by cross-linking antiparallel microtubules (3-5). The microtubule bundling functions of PRC1 play a critical role in maintaining structural integritry of the spindle midzone during cytokinesis (3,6,7).

  1. Pellman, D. et al. (1995) J Cell Biol 130, 1373-85.
  2. Jiang, W. et al. (1998) Mol Cell 2, 877-85.
  3. Kurasawa, Y. et al. (2004) EMBO J 23, 3237-48.
  4. Zhu, C. and Jiang, W. (2005) Proc Natl Acad Sci USA 102, 343-8.
  5. Subramanian, R. et al. (2010) Cell 142, 433-43.
  6. Mollinari, C. et al. (2002) J Cell Biol 157, 1175-86.
  7. Zhu, C. et al. (2006) Proc Natl Acad Sci USA 103, 6196-201.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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