Cell Signaling Technology

Product Pathways - Metabolism

AceCS1 (D19C6) Rabbit mAb #3658

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 78 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AceCS1 (D19C6) Rabbit mAb detects endogenous levels of total cytoplasmic acetyl-CoA synthetase (AceCS1).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human cytoplasmic acetyl-CoA synthetase (AceCS1).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using AceCS1 (D19C6) Rabbit mAb.

Background

Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA (1, 2). Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1, 2). Studies suggest that this enzyme is regulated by sterol regulatory element-binding proteins (2). It was also shown that this enzyme is acetylated by protein acetyltransferases in cells (3, 4). SIRT1 deacetylates this enzyme at Lys661 resulting in its activation and the increase of fatty acid synthesis (3, 4).

  1. Ikeda, Y. et al. (2001) J Biol Chem 276, 34259-69.
  2. Luong, A. et al. (2000) J Biol Chem 275, 26458-66.
  3. Hallows, W.C. et al. (2006) Proc Natl Acad Sci U S A 103, 10230-5.
  4. Schwer, B. and Verdin, E. (2008) Cell Metab 7, 104-12.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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