Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

WAVE-2 (D2C8) XP™ Rabbit mAb #3659

This XP™ monoclonal antibody was developed using our eXceptional Monoclonal Technology (XMT™).

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 84 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

WAVE-2 (D2C8) XP™ Rabbit mAb detects endogenous levels of total WAVE-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human WAVE-2.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using WAVE-2 (D2C8) XP™ Rabbit mAb.

IP

IP

Immunoprecipitation of WAVE-2 from HeLa cells using WAVE-2 (D2C8) XP™ Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 5% input.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or EGF-treated #9908 (right), using Wave-2 (D2C8) XP™ Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Wiscott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).

  1. Millard, T.H. et al. (2004) Biochem J. 380, 1-17.
  2. Yan, C. et al. (2003) EMBO J. 22, 3602-3612.
  3. Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904.
  4. Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132.
  5. Suetsugu, S. et al. (1999) Biochem. Biophys. Res. Commun. 260, 296-302.
  6. Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974.
  7. Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308, 135-145.
  8. Sossey-Alaoui, K. et al. (2007) Am J Pathol 170, 2112-21.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products