Cell Signaling Technology

Product Pathways - Glucose Metabolism

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661

Applications Reactivity MW (kDa) Source
W IP IHC-P IF-IC H M R Mk (C) (B) 280 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79. The antibody recognizes both ACCalpha and ACCbeta.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser79 of rat ACC. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells, untreated or oligomycin-treated, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or serum-starved (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.


IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, either untreated (left) or serum-starved and AICAR-treated (right), and labeled with Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα is the predominant isoform found in liver, adipocytes and mammary gland, while the 280 kDa ACCβ is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

  1. Ruderman, N.B. et al. (1999) Am. J. Physiol. 276, E1-E18.
  2. Ha, J. et al. (1994) J. Biol. Chem. 269, 22162-22168.
  3. Abu-Elheiga, L. et al. (2001) Science 291, 2613-2616.
  4. Levert, K.L. et al. (2002) J. Biol. Chem. 277, 16347-16350.

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