Product Pathways - Glucose Metabolism
Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P | H M R Mk (C) (B) | Endogenous | 280 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
B=Bovine
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79. The antibody recognizes both ACCalpha and ACCbeta.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser79 of rat ACC. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HEK293 cells, untreated or oligomycin-treated, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or serum-starved (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.
Background
Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).
- Ruderman, N.B. et al. (1999) Am. J. Physiol. 276, E1-E18.
- Ha, J. et al. (1994) J. Biol. Chem. 269, 22162-22168.
- Abu-Elheiga, L. et al. (2001) Science 291, 2613-2616.
- Levert, K.L. et al. (2002) J. Biol. Chem. 277, 16347-16350.
Application References
- Shaw, R. J. et al. (2004) The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress. PNAS 101 (10), 3329-3335. Applications: Western Blotting
- Pilon, G. et al. (2004) Inhibition of Inducible Nitric-oxide Synthase by Activators of AMP-activated Protein Kinase. The Journal of Biological Chemistry 279 (20), 20767-20774. Applications: Western Blotting
- Aymerich, I. et al. (2006) J Cell Sci 119, 1612-21. Applications: Western Blotting
- Goransson, O. et al. (2007) J Biol Chem 282, 32549-60. Applications: Western Blotting
- Gonzalez, A.A. et al. (2004) Am J Physiol Endocrinol Metab 287, E1032-7. Applications: Western Blotting
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
