Cell Signaling Technology

Product Pathways - Glucose Metabolism

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P H M R Mk (C) (B) Endogenous 280 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79. The antibody recognizes both ACCalpha and ACCbeta.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of rat ACC. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells, untreated or oligomycin-treated, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or serum-starved (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.


Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while the 280 kDa ACC2 (ACCβ) form is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.
  6. Hadad, S.M. et al. (2009) BMC Cancer 9, 307.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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