Cell Signaling Technology

Product Pathways - Metabolism

Acetyl-CoA Carboxylase Antibody #3662

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P IF-IC F H M R Mk B (C) (Dm) Endogenous 280 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  Dm=D. melanogaster  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Acetyl CoA Carboxylase Antibody detects endogenous levels of all isoforms of acetyl CoA carboxylase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys557 of human acetyl CoA carboxylase alpha1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293, HeLa, A431, NIH/3T3, L929, C6, H-4-IIE and BAEC cells, using Acetyl CoA Carboxylase Antibody.

IP

IP

Immunprecipitation of Acetyl-CoA Carboxylase from AICAR treated C2C12 cell extracts using Acetyl CoA Carboxylase antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Acetyl CoA Carboxylase Antibody in the presence of control peptide (left) or AcetylCoA Carboxylase Blocking Peptide #1062 (right).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells, using Acetyl CoA Carboxylase Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal microscopic images of A431 cells showing cytoplasmic stain with Acetyl CoA Carboxylase Antibody (A) compared to an isotype control (B).

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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