Product Pathways - Glucose Metabolism
Acetyl-CoA Carboxylase Antibody #3662
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk B (C) (Dr) | 280 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
B=Bovine
Dr=Drosophila
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Acetyl CoA Carboxylase Antibody detects endogenous levels of all isoforms of acetyl CoA carboxylase protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Lys557 of human acetyl CoA carboxylase alpha1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293, HeLa, A431, NIH/3T3, L929, C6, H-4-IIE and BAEC cells, using Acetyl CoA Carboxylase Antibody.
IP
Immunprecipitation of Acetyl-CoA Carboxylase from AICAR treated C2C12 cell extracts using Acetyl CoA Carboxylase antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Acetyl CoA Carboxylase Antibody in the presence of control peptide (left) or AcetylCoA Carboxylase Blocking Peptide #1062 (right).
Flow Cytometry
Flow cytometric analysis of untreated HeLa cells, using Acetyl CoA Carboxylase Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal microscopic images of A431 cells showing cytoplasmic stain with Acetyl CoA Carboxylase Antibody (A) compared to an isotype control (B).
Background
Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα is the predominant isoform found in liver, adipocytes and mammary gland, while the 280 kDa ACCβ is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).
- Ruderman, N.B. et al. (1999) Am. J. Physiol. 276, E1-E18.
- Ha, J. et al. (1994) J. Biol. Chem. 269, 22162-22168.
- Abu-Elheiga, L. et al. (2001) Science 291, 2613-2616.
- Levert, K.L. et al. (2002) J. Biol. Chem. 277, 16347-16350.
Application References
- Goransson, O. et al. (2007) J Biol Chem 282, 32549-60. This article references the use of Acetyl-CoA Carboxylase Antibody in the following applications: Western Blotting
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Companion Products
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- 2536 AMPKγ2 Antibody
- 9944 AICAR
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9158 AMPK Control Cell Extracts
- 1062 Acetyl-CoA Carboxylase Blocking Peptide
