Product Pathways - Wnt / Hedgehog / Notch
CD133 (C24B9) Rabbit mAb #3663
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P F | H | 133 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CD133 (C24B9) Rabbit mAb detects endogenous levels of total CD133 protein.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region (predicted to be extracellular) surrounding Asp562 of human CD133.
Western Blotting
Western blot analysis of total cell lysates from various cell types using CD133 (C24B9) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded CaCo-2 (left) or HeLa cells (right) using CD133 (C24B9) Rabbit mAb. Note: HeLa cells lack CD133.
Flow Cytometry
Flow cytometric analysis of unpermeabilized NCCIT cells using CD133 (C24B9) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Background
CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicated molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may results in retinal degeneration in humans (8).
- Yin, A.H. et al. (1997) Blood 90, 5002-5012.
- Miraglia, S. et al. (1997) Blood 90, 5013-5021.
- Handgretinger, R. et al. (2003) Ann. N.Y. Acad. Sci. 996, 141-151.
- Monzani, E. et al. (2007) Eur. J. Cancer 43, 935-946.
- O'Brien, C.A. et al. (2007) Nature 445, 106-110.
- Ricci-Vitiani, L. et al. (2007) Nature 445, 111-115.
- Singh, S.K. et al. (2004) Nature 432, 396-401.
- Maw, M.A. et al. (2000) Hum. Mol. Genet. 9, 27-34.
Application References
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