Product Pathways - Neuroscience
GFAP (GA5) Mouse mAb #3670
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-F | H M R | Endogenous | 50 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
GFAP (GA5) Mouse mAb detects endogenous levels of total GFAP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord.
Western Blotting
Western blot analysis of extracts from mouse and rat brain, using GFAP (GA5) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using GFAP (GA5) Mouse mAb.
IF-F
Confocal immunofluorescence image of rat hippocampus labeled with GFAP (GA5) Mouse mAb (red), Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green), and CREB (48H2) Rabbit mAb #9197 (blue).
Background
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in non-myelin forming Schwann cells in the peripheral nervous system (3).
- Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
- Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
- Jessen, K.R. et al. (1990) Development 109, 91-103.
Application References
- Davies, J.E. et al. (2008) J Biol 7, 24. Applications: IF-IC (In Cells)
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
