Cell Signaling Technology

Product Pathways - Neuroscience

GFAP (GA5) Mouse mAb #3670

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-F H M R Endogenous 50 Mouse IgG1

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GFAP (GA5) Mouse mAb detects endogenous levels of total GFAP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using GFAP (GA5) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using GFAP (GA5) Mouse mAb.

IF-F

IF-F

Confocal immunofluorescence image of rat hippocampus labeled with GFAP (GA5) Mouse mAb (red), Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green), and CREB (48H2) Rabbit mAb #9197 (blue).


Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3).

  1. Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
  2. Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
  3. Jessen, K.R. et al. (1990) Development 109, 91-103.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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