Product Pathways - Cytoskeletal Signaling

Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IF-IC	H M R Dm (Pg) (C) (B) (X) (Z)	Endogenous	18	Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Pg=Pig  C=Chicken  B=Bovine  Dm=D. melanogaster  X=Xenopus  Z=Zebra Fish
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Myosin Light Chain 2 (Ser19) Antibody detects endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. The antibody does not cross-react with the cardiac isoform of myosin light chain 2.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser19 of human myosin light chain 2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).

1. Ikebe, M. and Hartshorne, D.J. (1985) J. Biol. Chem. 260, 10027-10031.
2. Tan, J. L. et al. (1992) Annu. Rev. Biochem. 61, 721-759.
3. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
4. Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573.
5. Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605.
6. Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094.

Application References

• John, G. R. et al. (2004) Interleukin-1beta Induces a Reactive Astroglial Phenotype via Deactivation of the Rho GTPase-Rock Axis. J. Neurosci. 24 (11), 2837-2845. This article references the use of Phospho-Myosin Light Chain 2 (Ser19) Antibody in the following applications: IC-IF Western Blotting
• Iwabu, A. et al. (2004) Epidermal Growth Factor Induces Fibroblast Contractility and Motility via a Protein Kinase C delta-dependent Pathway. J. Biol. Chem. 279 (15), 14551-14560. This article references the use of Phospho-Myosin Light Chain 2 (Ser19) Antibody in the following applications: Western Blotting
• Kamijo, K. et al. (2006) Mol Biol Cell 17, 43-55. This article references the use of Phospho-Myosin Light Chain 2 (Ser19) Antibody in the following applications: IF-IC (In Cells)

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• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 3672 Myosin Light Chain 2 Antibody
• 3675 Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.