Product Pathways - Glucose Metabolism
Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Hm | Endogenous | 280 kDa | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Acetyl-CoA Carboxylase (C83B10) Rabbit mAb detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1.
Western Blotting
Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of 293 cells, using Acetyl-CoA Carboxylase (C83B10) Rb mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).
Background
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while the 280 kDa ACC2 (ACCβ) form is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).
- Castle, J.C. et al. (2009) PLoS One 4, e4369.
- Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
- Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
- Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
- Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.
Application References
- De Raedt, T. et al. (2011) Cancer Cell 20, 400-13. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3661 Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody
- 3662 Acetyl-CoA Carboxylase Antibody
- 4188 Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb
- 2535 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb
- 2531 Phospho-AMPKα (Thr172) Antibody
- 2532 AMPKα Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9944 AICAR
- 1062 Acetyl-CoA Carboxylase Blocking Peptide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
