Cell Signaling Technology

Product Pathways - Glucose Metabolism

Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676

Applications Reactivity MW (kDa) Source Isotype
W IHC-P IF-IC F H M R Hm 280 kDa Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Acetyl-CoA Carboxylase (C83B10) Rabbit mAb detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293 cells, using Acetyl-CoA Carboxylase (C83B10) Rb mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).

Background

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα is the predominant isoform found in liver, adipocytes and mammary gland, while the 280 kDa ACCβ is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

  1. Ruderman, N.B. et al. (1999) Am. J. Physiol. 276, E1-E18.
  2. Ha, J. et al. (1994) J. Biol. Chem. 269, 22162-22168.
  3. Abu-Elheiga, L. et al. (2001) Science 291, 2613-2616.
  4. Levert, K.L. et al. (2002) J. Biol. Chem. 277, 16347-16350.

Application References

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