Secondary Antibodies
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678
Protocols
- 3678:
- Western Blotting
Specificity / Sensitivity
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb recognizes the native rabbit IgG. It does not recognize the denatured and reduced rabbit IgG heavy (about 50 kDa) or light (about 25 kDa) chains on western blot.
Source / Purification
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Western Blotting
Western blot analysis of decreasing concentrations of total rabbit IgG, reduced and denatured in 1X SDS loading buffer with DTT, using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 (Panel B), or Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected using Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.
IP
Immunoprecipitation of PRAS40 from untreated HeLa cells using PRAS40 (D23C7) Rabbit mAb #2691 (Lane 2). All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody, including input control in lane 1. Secondary antibodies include Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 (Panel B), and Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected using Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.
Description
Affinity purified mouse anti-rabbit IgG (Conformation Specific) antibody.
Applications
This product has been optimized for use as a secondary antibody in Western blotting applications.
Background
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 3677 Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
