Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody #3681

Applications Reactivity Sensitivity MW (kDa) Source
W H M GP (R) (Dm) Endogenous 59 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Dm=D. melanogaster  GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody detects endogenous Mst1 and Mst2 only when phosphorylated at threonine 183 and threonine 180, respectively. The antibody may cross-react with phosphorylated Mst3 and Mst4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183 of human Mst1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from WEHI-231 cells and guinea pig neutrophils treated with staurosporine for the indicated times, using Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody (upper) or Mst1 Antibody #3682 (lower).

Background

Mst kinases, members of the STE20 family of kinases, are upstream activators of MAPK pathways that regulate processes such as apoptosis, morphogenesis and cytoskeletal rearrangements. The amino-terminal kinase domain of Mst is considerably homologous to the kinase domain of yeast STE20 kinase and other p21-activated kinases (1). The carboxy-terminal region of Mst1 and Mst2 contains dimerization and inhibitory domains (1-3). Dimerization and phosphorylation at the activation loop results in translocation of Mst1 from the cytosol to the nucleus (3). Growing evidence indicates that Mst1, Mst2 and Mst3 are activated by apoptotic signals as well as other stress conditions (4-6). Complete activation of Mst1 requires both phosphorylation and caspase-mediated cleavage (4). Sequence alignment of the activation loop of the GCK family indicates that Thr183 of Mst1 and Thr180 of Mst2 are the conserved residues and might be critical for the activity of the kinases. Activated Mst kinases may rely on p38 MAPK and JNK pathways to amplify apoptotic signals (5). Phosphorylation at Ser327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage (4).

  1. Dan, I. et al. (2001) Trends Cell Biol. 11, 220-230.
  2. Creasy, C.L. et al. (1996) J. Biol. Chem. 271, 21049-21053.
  3. Lee, K. and Yonehara, S. (2002) J. Biol. Chem. 277, 12351-12358.
  4. Graves, J.D. et al. (2001) J. Biol. Chem. 276, 14909-14915.
  5. Lee, K. et al. (2001) J. Biol. Chem. 276, 19276-19285.
  6. Graves, J.D. et al. (1998) EMBO J. 17, 2224-2234.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products