Product Pathways - Metabolism
GAPDH (14C10) Rabbit mAb (HRP Conjugate) #3683
PhosphoSitePlus® protein, site, and accession data: GAPDH
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 37 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3683:
- Western Blotting
Specificity / Sensitivity
GAPDH (14C10) Rabbit mAb (HRP Conjugate) detects endogenous levels of total GAPDH protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. The antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
Western Blotting
Western blot analysis of extracts from NIH/3T3, HeLa and C6 cells using GAPDH (14C10) Rabbit mAb (HRP Conjugate).
Description
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The unconjugated GAPDH (14C10) Rabbit mAb #2118 reacts with human, mouse, rat and monkey GAPDH. CST expects that GAPDH (14C10) (HRP conjugate) #3683 will also recognize GAPDH in these species.
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
- Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
- Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
- Zheng, L. et al. (2003) Cell 114, 255-66.
- Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
- Wang, Q. et al. (2005) FASEB J. 19, 869-71.
Application References
- Han, W. et al. (2011) PLoS One 6, e18691. Applications: Western Blotting
- Muller, F.L. et al. (2012) Nature 488, 337-42. Applications: Western Blotting
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Companion Products
- 2118 GAPDH (14C10) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
