Cell Signaling Technology

Product Pathways - Apoptosis

TRADD (7G8) Rabbit mAb #3684

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 32 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TRADD (7G8) Rabbit mAb detects endogenous levels of total TRADD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding glycine 227 of human TRADD.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7, SR, and A673 cell lines, using TRADD (7G8) Rabbit mAb.

Background

Apoptosis mediated by death factors like FasL and TNF-α involves the formation of a death-inducing signaling complex (DISC) to their respective receptors (1). Upon ligand activation to their receptors, Fas and TNF-R1 associate with death domain (DD) containing adaptor proteins FADD (Fas associated death domain) (2,3) and TRADD (TNF-R1 associated death domain) (4). In addition to its carboxy-terminal DD, FADD contains an amino-terminal death effector domain (DED) that binds to DEDs found on caspase-8 which leads to activation of this initiator caspase (5,6). Caspase-8 subsequently activates downstream effector caspases, like caspase-3, resulting in the cleavage of proteins involved in the execution of apoptosis. Unlike FADD, TRADD does not contain a DED (4). Apoptosis driven by TNF-R1 binding to TRADD involves association of TRADD and FADD which then leads to activation of caspase-8 (7).

  1. Nagata, S. (1997) Cell 88, 355-65.
  2. Chinnaiyan, A.M. et al. (1995) Cell 81, 505-12.
  3. Boldin, M.P. et al. (1995) J. Biol. Chem. 270, 7795-8.
  4. Hsu, H. et al. (1995) Cell 81, 495-504.
  5. Muzio, M. et al. (1996) Cell 85, 817-27.
  6. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  7. Hsu, H. et al. (1996) Cell 84, 299-308.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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