Product Pathways - Apoptosis / Autophagy
TRADD (7G8) Rabbit mAb #3684
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 32 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TRADD (7G8) Rabbit mAb detects endogenous levels of total TRADD protein.
Source / Purification
Rabbit monoclonal antibodies are produced from spleens taken from rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to residues surrounding glycine 227 of human TRADD.
Western Blotting
Western blot analysis of extracts from MCF7, SR, and A673 cell lines, using TRADD (7G8) Rabbit mAb.
Background
Apoptosis mediated by death factors like FasL and TNF-α involves the formation of a death-inducing signaling complex (DISC) to their respective receptors (1). Upon ligand activation to their receptors, Fas and TNF-R1 associate with death domain (DD) containing adaptor proteins FADD (Fas associated death domain) (2,3) and TRADD (TNF-R1 associated death domain) (4). In addition to its carboxy-terminal DD, FADD contains an amino-terminal death effector domain (DED) that binds to DEDs found on caspase-8 which leads to activation of this initiator caspase (5,6). Caspase-8 subsequently activates downstream effector caspases, like caspase-3, resulting in the cleavage of proteins involved in the execution of apoptosis. Unlike FADD, TRADD does not contain a DED (4). Apoptosis driven by TNF-R1 binding to TRADD involves association of TRADD and FADD which then leads to activation of caspase-8 (7).
- Nagata, S. (1997) Cell 88, 355-65.
- Chinnaiyan, A.M. et al. (1995) Cell 81, 505-12.
- Boldin, M.P. et al. (1995) J. Biol. Chem. 270, 7795-8.
- Hsu, H. et al. (1995) Cell 81, 495-504.
- Muzio, M. et al. (1996) Cell 85, 817-27.
- Boldin, M.P. et al. (1996) Cell 85, 803-15.
- Hsu, H. et al. (1996) Cell 84, 299-308.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
