Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TCEB3/Elongin A Antibody #3685

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 100 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TCEB3/Elongin A Antibody recognizes endogenous levels of total TCEB3/Elongin A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCEB3/Elongin A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TCEB3/Elongin A Antibody.

Background

The Elongin complex is a heterotrimer composed of TCEB3/Elongin A, Elongin B (TCEB2), and Elongin C (TCEB1) subunits (1-3). The Elongin complex regulates the rate of RNA polymerase II (RNAPII) transcription elongation by releasing the transient pausing of RNAPII at multiple sites along the DNA. TCEB3/Elongin A is the transcriptionally active subunit, while Elongin B and C subunits play a regulatory role (3,4). TCEB3/Elongin A may be required for expression of a subset of cell cycle regulated genes, and embryonic stem (ES) cells lacking TCEB3/Elongin A show abnormalities in cell size, growth, and cell cycle distribution (5). In addition, the Elongin complex has been shown to interact with the cullin family and RING finger proteins Cul5/Rbx2 upon UV-induced DNA damage, removing arrested RNAPII at sites of DNA damage by ubiquitination and degradation as part of an E3 ubiquitin ligase (6).

  1. Bradsher, J.N. et al. (1993) J Biol Chem 268, 25587-93.
  2. Garrett, K.P. et al. (1994) Proc Natl Acad Sci U S A 91, 5237-41.
  3. Aso, T. et al. (1995) Science 269, 1439-43.
  4. Aso, T. et al. (1996) EMBO J 15, 5557-66.
  5. Yamazaki, K. et al. (2003) J Biol Chem 278, 13585-9.
  6. Yasukawa, T. et al. (2008) EMBO J 27, 3256-66.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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