Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

p27 Kip1 (D69C12) XP™ Rabbit mAb #3686

This XP™ monoclonal antibody was developed using our eXceptional Monoclonal Technology (XMT™).

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H R Mk Endogenous 27 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

p27 Kip1 (D69C12) XP™ Rabbit mAb detects endogenous levels of total p27 Kip1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p27 Kip1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using p27 Kip1 (D69C12) XP™ Rabbit mAb.

IP

IP

Immunoprecipitation of p27 Kip1 from 293 cells using p27 Kip1 (D69C12) XP™ Rabbit mAb. Western analysis was performed using the same antibody. Lane 1 is 5% input.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using p27 Kip1 (D69C12) XP™ Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


Background

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).

  1. Lloyd, R.V. et al. (1999) Am. J. Pathol. 154, 313-323.
  2. Polyak, K. et al. (1994) Genes Dev. 8, 9-22.
  3. Kato, J.Y. et al. (1994) Cell 79, 487-496.
  4. Vlach, J. et al. (1997) EMBO J. 16, 5334-5344.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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