Product Pathways - Cell Cycle / Checkpoint
p27 Kip1 (D69C12) XP® Rabbit mAb #3686
|3686S||100 µl (10 western blots)||---||In Stock||---|
|3686P||40 µl (4 western blots)||---||In Stock||---|
|3686||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Rat, Monkey||Endogenous||27||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
p27 Kip1 (D69C12) XP® Rabbit mAb detects endogenous levels of total p27 Kip1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p27 Kip1.
Western blot analysis of extracts from various cell types using p27 Kip1 (D69C12) XP® Rabbit mAb.
Immunoprecipitation of p27 Kip1 from 293 cells using p27 Kip1 (D69C12) XP® Rabbit mAb. Western analysis was performed using the same antibody. Lane 1 is 5% input.
Flow cytometric analysis of Jurkat cells using p27 Kip1 (D69C12) XP® Rabbit mAb versus Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).
- Stein, C. et al. (2012) Nucleic Acids Res , . Applications: Western Blotting.
- Poindessous, V. et al. (2011) Clin Cancer Res 17, 6522-30. Applications: Western Blotting.
- Yang, S. et al. (2011) PLoS One 6, e26343. Applications: Western Blotting.
- Negrotto, S. et al. (2011) Cancer Res 71, 1431-41. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.