Product Pathways - Cell Cycle / Checkpoint
p27 Kip1 (D37H1) Rabbit mAb #3688
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PhosphoSitePlus® protein, site, and accession data: p27Kip1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 27 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
p27 Kip1 (D37H1) Rabbit mAb detects endogenous levels of total p27 Kip1 protein. The antibody may cross-react with p57 Kip2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p27 Kip1.
Western Blotting
Western blot analysis of extracts from various cell types using p27 Kip1 (D37H1) Rabbit mAb.
IP
Immunoprecipitation of p27 Kip1 from NIH/3T3 cells using p27 Kip1 (D37H1) Rabbit mAb. Western blot analysis was performed using the same antibody. Lane 1 is 5% input.
Background
p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).
- Lloyd, R.V. et al. (1999) Am. J. Pathol. 154, 313-323.
- Polyak, K. et al. (1994) Genes Dev. 8, 9-22.
- Kato, J.Y. et al. (1994) Cell 79, 487-496.
- Vlach, J. et al. (1997) EMBO J. 16, 5334-5344.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
