Cell Signaling Technology

Product Pathways - Glucose Metabolism

Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3689

Applications Reactivity Sensitivity Isotype
IF-IC F H M Endogenous Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total hexokinase I protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF-7 cells using Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Hexokinase I (C35C4) Rabbit mAb #2024.

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

  1. Warburg, O. (1956) Science 123, 309-314.
  2. Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
  3. Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 from Epitomics, Inc.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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