Product Pathways - Protein Translation
Phospho-eEF2k (Ser366) Antibody #3691
|3691S||100 µl (10 western blots)||---||In Stock||---|
|3691P||40 µl (4 western blots)||---||In Stock||---|
|3691||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Rat, Monkey||Endogenous||105||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-eEF2k (Ser366) Antibody detects endogenous levels of eEF2k only when phosphorylated at Ser366.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody #3692 (lower).
Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.
- Ryazanov, A.G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4884-4889.
- Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7839-7943.
- Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792.
- Redpath, N.T. et al. (1993) Biochem. J. 293, 31-34.
- Diggle, T.A. et al. (1998) Biochem. J. 336, 525-529.
- Hovland, R. et al. (1999) FEBS Lett. 444, 97-101.
- Wang, X. et al. (2001) EMBO J. 20, 4370-4379.
- Knebel, A. et al. (2001) EMBO J. 20, 4360-4369.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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