Cell Signaling Technology

Product Pathways - Apoptosis

Perforin Antibody (Mouse Specific) #3693

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC F M Endogenous 70-75 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Perforin Antibody (Mouse Specific) detects endogenous levels of total Perforin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding leucine 349 of mouse perforin.

Western Blotting

Western Blotting

Western blot analysis of extracts from CTLL-2 cells, using Perforin Antibody (Mouse Specific).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated CTLL-2 cells, using Perforin Antibody (Mouse Specific) (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Immunofluorescent analysis of CTLL-2 cells showing granule staining, using Perforin Antibody (Mouse Specific).


Background

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).

Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.

  1. Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014.
  2. Lord, S.J. et al. (2003) Immunol. Rev. 193, 31-8.
  3. Trapani, J.A. and Sutton, V.R. (2003) Curr. Opin. Immunol. 15, 533-43.
  4. Catalfamo, M. and Henkart, P.A. (2003) Curr. Opin. Immunol. 15, 522-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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