Cell Signaling Technology

Product Pathways - Apoptosis

TRADD Antibody #3694

Applications Reactivity Sensitivity MW (kDa) Source
W IP F H M R Mk Endogenous 34 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TRADD Antibody detects endogenous levels of total TRADD protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 138 of human TRADD. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7 (human), C2C12 (mouse) and KNRK (rat) cell lines, using TRADD Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT29 cells, using TRADD antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Apoptosis mediated by death factors like FasL and TNF-α involves the formation of a death-inducing signaling complex (DISC) to their respective receptors (1). Upon ligand activation to their receptors, Fas and TNF-R1 associate with death domain (DD) containing adaptor proteins FADD (Fas associated death domain) (2,3) and TRADD (TNF-R1 associated death domain) (4). In addition to its carboxy-terminal DD, FADD contains an amino-terminal death effector domain (DED) that binds to DEDs found on caspase-8 which leads to activation of this initiator caspase (5,6). Caspase-8 subsequently activates downstream effector caspases, like caspase-3, resulting in the cleavage of proteins involved in the execution of apoptosis. Unlike FADD, TRADD does not contain a DED (4). Apoptosis driven by TNF-R1 binding to TRADD involves association of TRADD and FADD which then leads to activation of caspase-8 (7).

  1. Nagata, S. (1997) Cell 88, 355-65.
  2. Chinnaiyan, A.M. et al. (1995) Cell 81, 505-12.
  3. Boldin, M.P. et al. (1995) J. Biol. Chem. 270, 7795-8.
  4. Hsu, H. et al. (1995) Cell 81, 495-504.
  5. Muzio, M. et al. (1996) Cell 85, 817-27.
  6. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  7. Hsu, H. et al. (1996) Cell 84, 299-308.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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