Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lineage Markers

LIN28A (D84C11) XP® Rabbit mAb #3695

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H (R) (Mk) Endogenous 26 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LIN28A (D84C11) XP® Rabbit mAb detects endogenous levels of total LIN28A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence at the carboxy terminus of human LIN28A.

Western Blotting

Western Blotting

Western blot analysis of extracts of NTERA-2 and IGROV1 cells using LIN28A (D84C11) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using LIN28A (D84C11) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells using Lin28A (D84C11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as ras, myc, and Hmga2 (3). It has recently been shown that LIN28A and LIN28B are upregulated in primary human tumors and in human cancer cell lines, their overexpression directly correlating to downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5) and allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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