Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-GRB10 (Tyr67) Antibody #3701
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Transfected Only | 76 (Transfected only) | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-GRB10 (Tyr67) Antibody detects transfected GRB10 only when phosphorylated at tyrosine 67. The antibody does not cross-react with phosphorylated GRB7 or GRB14.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr67 of human GRB10. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or transfected with either wild-type GRB10 (lanes 2-4) or GRB10 Y67G mutant (lanes 5-7), untreated or treated with EGF or insulin as indicated, using Phospho-GRB10 (Tyr67) Antibody (upper) or GRB10 Antibody #3702 (lower). (Plasmids provided by Dr. F. Liu, Dept. of Pharmacology and Biochemistry, University of Texas Health Science Center.)
Background
The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules such as Raf, Akt and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4).
- Han, D. C. et al. (2001) Oncogene 20, 6315-6321.
- Jahn, T. et al. (2002) Mol. Cell. Biol. 22, 979-991.
- Ooi, J. et al. (1995) Oncogene 10, 1621-1630.
- Langlais, P. et al. (2000) Oncogene 19, 2895-2903.
Application References
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Companion Products
- 3702 GRB10 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
