Product Pathways - NF-kB Signaling
TNF-α Antibody #3707
|3707S||100 µl (10 western blots)||---||In Stock||---|
|3707||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||17 mature. 25 precursor.||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
TNF-α Antibody detects endogenous levels of mature TNF-α as well TNF-α precursor protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly198 of human TNF-α protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 µg/ml, 18 hr; +), using TNF-α Antibody.
TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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