Product Pathways - MAPK Signaling
JNK1 (2C6) Mouse mAb #3708
|W||H M R Mk||Endogenous||46, 54||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
JNK1 (2C6) Mouse mAb detects endogenous levels of total JNK1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the amino terminus of human JNK1.
Western blot analysis of HeLa cell extracts, untransfected (lane 1), mock-transfected (lane 2) or transfected with SignalSilence® SAPK/JNK siRNA I #6232 (lane 3) or SignalSilence® SAPK/JNK siRNA II #6233 (lane 4) for 72 hours, using JNK1 (2C6) Mouse mAb.
The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).
- Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
- Ichijo, H. (1999) Oncogene 18, 6087-93.
- Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
- Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
- Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9258 SAPK/JNK (56G8) Rabbit mAb
- 9252 SAPK/JNK Antibody
- 4668 Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
- 4671 Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
- 9255 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
- 9257 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 4672 JNK2 Antibody
- 2305 JNK3 (55A8) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.