Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

TGF-β Receptor I Antibody #3712

Applications Reactivity Sensitivity MW (kDa) Source
W H R Mi (Pg) Endogenous 52 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  R=Rat  Mi=Mink  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TGF-β Receptor I Antibody detects endogenous levels of total TGF-β receptor I protein independent of phosphorylation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central intracellular region of human TGF-β receptor I. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Mv 1 Lu and Hep G2 cells using TGF-β Receptor I Antibody.

Background

Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).

  1. Massague, J. et al. (2000) Cell 103, 295-309.
  2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
  3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
  4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
  5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
  6. López-Casillas, F. et al. (1991) Cell 67, 785-795.
  7. Wang, X.F. et al. (1991) Cell 67, 797-805.
  8. Derynck, R. et al. (1998) Cell 95, 737-740.
  9. Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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