Cell Signaling Technology

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GST (26H1) Mouse mAb (Alexa Fluor® 555 Conjugate) #3720

Applications Reactivity Sensitivity Isotype
IF-IC All Transfected Only Mouse IgG2a

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

GST (26H1) Mouse mAb (Alexa Fluor® 555 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST fusion protein. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio 2-6.

IF-IC

IF-IC

Confocal immunofluorescent analysis of COS cells transiently transfected with a GST-tagged fusion protein using GST (26H1) Mouse mAb (Alexa Fluor® 555 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescence in cells transfected with GST-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).

  1. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
  2. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
  3. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

Application References

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Companion Products

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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