Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Pin1 (Ser16) Antibody #3721

Applications Reactivity Sensitivity MW (kDa) Source
W R (H) (M) (X) Endogenous 18 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Pin1 (Ser16) Antibody detects endogenous levels of Pin1 only when phosphorylated at serine 16.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser16 of human Pin1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 cells, untreated or treated with 0.1uM calyculin A #9902, using Phospho-Pin1 (Ser16) Antibody (upper) or Pin1 Antibody #3722 (lower).

Background

Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).

Phosphorylation of pin1 at Ser16 affects its subcellular localization and the ability of its WW domain to interact with potential targets (10).

  1. Lu, P. J. et al. (1999) Science 283, 1325-1328.
  2. Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643.
  3. Lu, K. P. et al. (1996) Nature 380, 544-547.
  4. Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883.
  5. Wu, X. et al. (2000) EMBO J. 19, 3727-3738.
  6. Winkler, K. E. et al. (2000) Science 287, 1644-1647.
  7. Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327.
  8. Shen, M. et al. (1998) Genes Dev. 12, 706-720.
  9. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
  10. Lu, P. J. et al. (2002) J Biol Chem 277, 2381-2384.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products