Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Pin1 Antibody #3722

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk (X) Endogenous 18 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  X=Xenopus
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Pin1 Antibody detects endogenous levels of total Pin1 protein. The antibody does not cross-react with other proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminal residues of human Pin1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using Pin1 Antibody.

Background

Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).

  1. Lu, P. J. et al. (1999) Science 283, 1325-1328.
  2. Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643.
  3. Lu, K. P. et al. (1996) Nature 380, 544-547.
  4. Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883.
  5. Wu, X. et al. (2000) EMBO J. 19, 3727-3738.
  6. Winkler, K. E. et al. (2000) Science 287, 1644-1647.
  7. Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327.
  8. Shen, M. et al. (1998) Genes Dev. 12, 706-720.
  9. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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