Product Pathways - Cell Cycle / Checkpoint
Pin1 Antibody #3722
PhosphoSitePlus® protein, site, and accession data: Pin1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk (X) | Endogenous | 18 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3722:
- Western Blotting
Specificity / Sensitivity
Pin1 Antibody detects endogenous levels of total Pin1 protein. The antibody does not cross-react with other proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human Pin1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using Pin1 Antibody.
Background
Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).
- Lu, P. J. et al. (1999) Science 283, 1325-1328.
- Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643.
- Lu, K. P. et al. (1996) Nature 380, 544-547.
- Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883.
- Wu, X. et al. (2000) EMBO J. 19, 3727-3738.
- Winkler, K. E. et al. (2000) Science 287, 1644-1647.
- Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327.
- Shen, M. et al. (1998) Genes Dev. 12, 706-720.
- Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
Application References
- Mannava, S. et al. (2011) Oncogene , . Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
