Cell Signaling Technology

Product Pathways - NF-kappaB Signaling

TNF-R2 Antibody #3727

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R (Mk) Endogenous 75 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

TNF-R2 Antibody detects endogenous levels of total TNF-R2 protein.

Source / Purification

Polyclonal antibodies are prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Asp335 within the intracellular region of human TNF-R2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from SW620, NK-92 and SR cell lines, using TNF-R2 Antibody.

Background

TNF-α is an important cytokine produced by numerous cell types including neutrophils, activated lymphocytes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3).

The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act throught TNF-R1 (6,7). In contrast, TNF-R2 appears to be important in T cell signaling and responses to infection (7,8). TNF-R2 binds to distinct members of the TRAF family leading to the activation of NF-κB (9,10). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (11,12).

  1. Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
  2. Locksley, R.M. et al. (2001) Cell 104, 487-501.
  3. Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
  4. Tartaglia, L.A. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 9292-9296.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160, 943-952.
  6. Tartaglia, L.A. et al. (1993) Cell 73, 213-216.
  7. Rothe, J. et al. (1993) Nature 364, 798-802.
  8. Aspalter, R.M. et al. (2003) J. Leukoc. Biol. 74, 572-582.
  9. Rothe, M. et al. (1994) Cell 78, 681-692.
  10. Rothe, M. et al. (1995) Science 269, 1424-1427.
  11. Humbert, M. et al. (1994) Am. J. Respir. Crit. Care Med. 149, 1681-1685.
  12. Schroder, J. et al. (1995) Infection 23, 143-8.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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