Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

GP130 Antibody #3732

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Endogenous 130 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GP130 Antibody detects endogenous levels of total GP130 protein independent of phosphorylation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GP130. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, PC12, COS and HeLa cells, using GP130 Antibody.

Background

GP130 is a signal-transducing subunit shared by the receptors for the IL-6 family of cytokines (1-3). The binding of a ligand to its receptor induces the dimerization of GP130, leading to activation of the Jak tyrosine kinase and to tyrosine phosphorylation of GP130. These events lead to the activation of multiple signal-transduction pathways, such as the Stat, Ras-MAPK and PI3 kinase pathways, whose activation is controlled by distinct regions of GP130 (4-7).

  1. Taga, T. (1996) J. Neurochem. 67, 1-10.
  2. Taga, T. (1997) Ann. Med. 29, 63-72.
  3. Taga, T. and Kishimoto, T. (1997) Annu. Rev. Immunol. 15, 797-819.
  4. Heinrich, P.C. et al. (1998) Biochem. J. 334, 297-314.
  5. Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.
  6. Kurth, I. et al. (1999) J. Immunol. 162, 1480-1487.
  7. Paonessa, G. et al. (1995) EMBO J. 14, 1942-1951.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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