Cell Signaling Technology

Product Pathways - Neuroscience

SAP102 (G670) Antibody #3733

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 102 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SAP102 (G670) Antibody detects endogenous levels of total SAP102 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding residue Gly670 of human SAP102. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat and mouse brain using SAP102 (G670) Antibody.

Background

Synapse-associated protein 102 (SAP102, DLG3) belongs to the membrane-associated guanylate kinase (MAGUK) protein family and is a homolog of the Drosophila disc large (dlg) tumor suppressor protein. SAP102 consists of three PDZ domains, a Src homology 3 (SH3) domain and a guanylate kinase (GK) domain (1). The SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth. SAP102 interacts with the carboxy terminus of the adenomatous polyposis coli (APC) tumor suppressor protein. Furthermore, SAP102 associates with PSD95 in the presence of calcium while the SH3 domain of SAP102 binds calmodulin (2,3). All three PDZ domains of SAP102 participate in binding to the NMDA receptor, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). This SAP102-NR2B interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the Erk/MAPK pathway (1,4). Neuronal SAP102 is concentrated at dendritic shafts and spines, axons, and synaptic junctions. At excitatory synapses, SAP102 is involved in NMDA receptor clustering and immobilization and links NMDA receptors to the submembraneous cytomatrix (4). SAP102 and the NMDA receptor function together to mediate plasticity, behavior, and signal transduction (1). A nonsyndromic form of X-linked mental retardation is caused by loss-of-function mutations to the SAP102 gene. The SAP102 protein may be involved in autism since MAGUK proteins in the NMDA receptor complex bind directly to the autism susceptibility gene neuroligin (1,5).

  1. Cuthbert, P.C. et al. (2007) J Neurosci 27, 2673-82.
  2. Makino, K. et al. (1997) Oncogene 14, 2425-33.
  3. Masuko, N. et al. (1999) J Biol Chem 274, 5782-90.
  4. Lau, L.F. et al. (1996) J Biol Chem 271, 21622-8.
  5. Yan, J. et al. (2005) Mol Psychiatry 10, 329-32.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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