Cell Signaling Technology

Product Pathways - NF-kB Signaling

TNF-R1 (C25C1) Rabbit mAb #3736

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 55 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TNF-R1 (C25C1) Rabbit mAb detects endogenous levels of human TNF-R1 protein. This antibody does not appear to cross-react with other related family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues within the intracellular region and surrounding Ser331 of human TNF-R1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549, LN-18 and HeLa cells using TNF-R1 (C25C1) Rabbit mAb.

Background

TNF-α is an important cytokine produced by numerous cell types including neutrophils, activated lymphoctyes, macrophages an NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3).

The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7). Cytotoxicity is mediated by a "death domain" with the intracellular region of the receptor that binds to the death domain adaptor protein TRADD and triggers the activation of caspases (8). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (9,10).

  1. Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
  2. Locksley, R.M. et al. (2001) Cell 104, 487-501.
  3. Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
  4. Tartaglia, L.A. et al. (1991) Proc Natl Acad Sci USA 88, 9292-6.
  5. Peschon, J.J. et al. (1998) J Immunol 160, 943-52.
  6. Tartaglia, L.A. et al. (1993) Cell 73, 213-6.
  7. Rothe, J. et al. (1993) Nature 364, 798-802.
  8. Chen, G. and Goeddel, D.V. (2002) Science 296, 1634-5.
  9. Humbert, M. et al. (1994) Am J Respir Crit Care Med 149, 1681-5.
  10. Schröder, J. et al. Infection 23, 143-8.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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