Product Pathways - NF-kappaB Signaling
TNF-R1 (C25C1) Rabbit mAb #3736
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 55 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TNF-R1 (C25C1) Rabbit mAb detects endogenous levels of human TNF-R1. This antibody does not appear to cross-react with other related family members.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues within the intracellular region and surrounding Ser331 of human TNF-R1 .
Western Blotting
Western blot analysis of extracts from A549, LN18 and HeLa cells using TNF-R1 (C25C1) Rabbit mAb.
Background
TNF-α is an important cytokine produced by numerous cell types including neutrophils, activated lymphocytes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3).
The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7). Cytotoxicity is mediated by a "death domain" with the intracellular region of the receptor that binds to the death domain adaptor protein TRADD and triggers the activation of caspases (8). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (9,10).
- Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
- Locksley, R.M. et al. (2001) Cell 104, 487-501.
- Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
- Tartaglia, L.A. et al. (1991) Proc Natl Acad Sci USA 88, 9292-6.
- Peschon, J.J. et al. (1998) J Immunol 160, 943-52.
- Tartaglia, L.A. et al. (1993) Cell 73, 213-6.
- Rothe, J. et al. (1993) Nature 364, 798-802.
- Chen, G. and Goeddel, D.V. (2002) Science 296, 1634-5.
- Humbert, M. et al. (1994) Am J Respir Crit Care Med 149, 1681-5.
- Schroder, J. et al. Infection 23, 143-8.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
