Product Pathways - Apoptosis

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Beclin-1 Antibody #3738

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R	Endogenous	60	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3738:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Beclin-1 Antibody detects endogenous levels of total Beclin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding threonine 72 of human Beclin-1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Kametaka, S. et al. (1998) J Biol Chem 273, 22284-91.
3. Liang, X.H. et al. (1999) Nature 402, 672-6.
4. Liang, X.H. et al. (1998) J Virol 72, 8586-96.
5. Aita, V.M. et al. (1999) Genomics 59, 59-65.
6. Liang, X.H. et al. (2001) Cancer Res 61, 3443-9.
7. Yue, Z. et al. (2003) Proc Natl Acad Sci USA 100, 15077-82.

Application References

• Shi, C.S. and Kehrl, J.H. (2008) J Biol Chem 283, 33175-82. Applications: Western Blotting
• Yang, J. et al. (2010) J Cell Sci 123, 861-70. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3495 Beclin-1 (D40C5) Rabbit mAb
• 4445 Autophagy Antibody Sampler Kit
• 6222 SignalSilence^® Beclin-1 siRNA I
• 6246 SignalSilence^® Beclin-1 siRNA II
• 9942 Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit
• 9941 Pro-Survival Bcl-2 Family Antibody Sampler Kit
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.