Product Pathways - Apoptosis / Autophagy
Beclin-1 Antibody #3738
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M R | Endogenous | 60 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Beclin-1 Antibody detects endogenous levels of total Beclin-1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding threonine 72 of human Beclin-1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Raji (human), C6 (rat) and C2C12 (mouse) cell lines, using Beclin-1 Antibody.
IC-IF
Immunofluorescent staining of HeLa cells, using Beclin-1 Antibody.
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast-two hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). It is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontanteous tumors. Stem cells from the null mice demonstrate an altered autophagic response although responses to apoptosis appeared normal (7). Overexpression of Beclin-1 in virally infected neurons in vivo resulted in signficant protection against Sindbis virus-induced disease and neuronal apoptosis (4).
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryotic Cell 1, 11-21.
- Kametaka, S. et al. (1998) J. Biol. Chem. 273, 22284-22291.
- Liang, X. H. et al. (1999) Nature 402, 672-676.
- Liang, X. H. et al. (1998) J. Virol. 72, 8586-8596.
- Aita, V. M. et al. (1999) Genomics 59, 59-65.
- Liang, X. H. et al. (2001) Cancer Res. 61, 3443-3449.
- Yue, Z. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 15077-15082.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
