Cell Signaling Technology

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Myc-Tag (9B11) Mouse mAb (PE Conjugate) #3739

Applications Reactivity Sensitivity Isotype
F All Transfected Only Mouse IgG2a

Applications Key:  F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb (PE Conjugate) detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS-7 cells, untreated (blue) or Myc transfected (green), using Myc-Tag (9B11) Mouse mAb (PE Conjugate).

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry in cells transfected with Myc-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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